Frozen embryos should be retrieved by the investigator one week post-freezing for long-term storage in liquid nitrogen in the investigator's own lab. Quality control is assured by freezing and thawing a representative sample of wild-type frozen embryos along with each line frozen down. A controlled-rate methanol bath freezer is used to gradually cool the embryos to -80☌, before plunging them into liquid nitrogen for storage. Another advantage is that during the process of embryo collection, most pathogenic organisms are excluded as in other methods used to rederive lines of mice.īriefly, the procedure entails harvesting embryos from donor females at the 8-cell developmental stage that is optimal for cryopreservation by our method. Mouse embryos stored in liquid nitrogen offer a safe way to preserve lines with potential future use but no current use and offer savings in facility space and the expense associated with keeping a breeding colony. Genetic banking through the use of mouse embryo cryopreservation is a practical means to store scientifically valuable mice. Once dams are deemed clean, then pups are transferred to destination vivarium.Pups are weaned and the dams are tested for pathogens.Recipient females are retained in CMM-E quarantine until new pups are weaned.Core collects embryos, washes them and transfers into the oviducts of pseudo-pregnant recipient females.Core super-ovulates and RIU personnel mate females with males in RIU.Core acquires wild-type females of the appropriate background strain and schedules procedure.Client coordinates the receipt of at least two males ages between 2 to 5 months, of desired strain into the RIU with the Animal Care Program Acquisitions Office.Embryos are harvested in the RIU, washed and transferred into pathogen free, pseudopregnant recipient females by the core, pups are transferred to client after clean bill of health. Males arrive in the rodent isolation unit (RIU), and wild-type females of the desired background strain are superovulated and mated to the males. Clinical Trials Resources for ResearchersĮmbryo Rederivation is used to create a pathogen free strain of mice from lines that arrive at the university from a non-approved vendor and mice contaminated with various pathogens (MHV, Pinworm, Parvovirus).Photoacoustic Imaging - Ultrasound Imaging.Two Science Enrichment Education Programs.
Hematology /Oncology Fellowship Program.Growth Regulation and Oncogenesis Training Program.Cancer Therapeutics Training (CT2) Program.San Diego Center for Precision Immunotherapy (SDCPI).Protocol Review and Monitoring System (PRMS).
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